Dilution Factor Calculator

Summary

The Dilution Factor Calculator computes the dilution factor (DF) between a stock and a working solution, determines the final concentration after one or more dilution steps, and generates a complete serial dilution table. Enter your stock concentration, desired final concentration, or dilution ratio to get instant results with a step-by-step breakdown.

How it works

1. Enter the stock concentration and its unit (M, mM, µM, nM, or %).
2. Choose a mode: single dilution or serial dilution.
3. For single dilution: enter the final volume and either the dilution factor or desired final concentration.
4. For serial dilution: enter the dilution factor per step and number of steps.
5. The calculator shows volumes to add, total volume at each step, and the concentration after each dilution.
6. Copy the full dilution table to your clipboard for use in lab notebooks.

Use cases

• Preparing working solutions from a concentrated stock in molecular biology.
• Setting up 10-fold or 2-fold serial dilutions for standard curves.
• Calculating how much diluent to add to reach a target concentration.
• Designing dilution series for ELISA, qPCR, or cell-based assays.
• Teaching lab students to understand dilution ratios and concentration math.
• Verifying dilution schemes before performing expensive reagent preparations.

Frequently Asked Questions