DNA/RNA Ratio Calculator

Summary

Enter A260 absorbance values for your DNA and RNA samples to calculate the DNA:RNA ratio instantly.

How it works

1. Enter the DNA concentration or A260 absorbance value for your DNA sample.
2. Enter the RNA concentration or A260 absorbance value for your RNA sample.
3. Select whether you are entering raw A260 readings or pre-calculated concentrations (ng/µL).
4. The calculator converts A260 values using standard extinction factors (50 ng/µL per A260 unit for dsDNA, 40 ng/µL for RNA).
5. The DNA:RNA ratio is displayed along with the individual concentrations.
6. Use the batch mode to evaluate multiple samples at once.

Use cases

• Quality-check total RNA extractions before downstream assays.
• Assess DNA contamination in RNA preparations.
• Evaluate RNA depletion efficiency in ribosomal RNA removal protocols.
• Monitor DNA:RNA balance in chromatin-associated RNA studies.
• Verify co-extraction ratios in simultaneous DNA/RNA isolation kits.
• Document nucleic acid ratios for experimental metadata.

Frequently Asked Questions