HPLC Sample Dilution Calculator

Summary

Enter your stock concentration, target concentration, and final volume to get the exact volumes of stock solution and diluent needed for HPLC sample preparation.

How it works

1. Enter the stock solution concentration (C1) and select its unit.
2. Enter the target HPLC sample concentration (C2) in the same or equivalent unit.
3. Enter the total final volume (V2) you need to prepare and its unit.
4. Click Calculate — the tool solves V1 = (C2 × V2) / C1.
5. Read the stock volume (V1) to pipette and the diluent volume to add.
6. Use the dilution factor displayed to verify your preparation is within the linear range.

Use cases

• Preparing calibration standards at specific concentrations from a concentrated stock.
• Diluting a sample extract to fall within the HPLC detector linear range.
• Calculating how much of a reference standard to add to a fixed-volume vial.
• Working out diluent volumes when scaling sample prep up or down.
• Verifying that a dilution factor is achievable with the pipettes available.
• Quickly recalculating when a different final volume or concentration is needed.

Frequently Asked Questions