Restriction Enzyme Finder

Summary

Paste a DNA sequence to find all restriction enzyme cut sites from a library of 40+ common enzymes.

How it works

1. Paste or type your DNA sequence into the input box.
2. Select which enzyme groups to include — popular cloning enzymes are checked by default.
3. The tool scans the sequence and lists every enzyme that cuts, along with the number of cut sites and their positions.
4. Click any enzyme row to see the cut positions highlighted in the sequence.
5. Use the filter to search for a specific enzyme by name.
6. Copy the results table to your clipboard for use in a lab notebook.

Use cases

• Screen a cloning insert for compatible restriction sites.
• Design a restriction digest map for gel verification.
• Identify unique (single-cut) sites for linearizing a plasmid.
• Find enzymes that do NOT cut your insert before cloning.
• Quickly verify a published sequence by checking expected cut sites.
• Select compatible cohesive ends for directional ligation.
• Teach restriction enzyme biology with a hands-on visual tool.

Frequently Asked Questions